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Mouse nhpESC chimeras were also prepared using the
2018-10-22

Mouse–nhpESC chimeras were also prepared using the mouse tetraploid complementation assay (Nagy, 2003). We electrofused mouse 2-cell embryos to produce 4N embryos and permitted these to develop to the expanded blastocyst stage before injecting them with GFP-expressing nhpESCs and performing embryo t
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AZD6244 We conclude that we have established xeno free fibro
2018-10-22

We conclude, that we have established xeno-free fibroblast feeder cell lines using HS-containing culture medium and, in accordance with published data agree, that HS is a feasible substitute for FBS (Richards et al., 2003; Kibschull et al., 2011; Ellerstrom et al., 2006; Meng et al., 2008; Unger et
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mg115 Supplier Human mesenchymal stem cells hMSCs
2018-10-22

Human mesenchymal stem mg115 Supplier (hMSCs) are isolated firstly from bone marrow (BM) (Friedenstein et al., 1976; Caplan, 1991) and isolated subsequently from other tissues such as adipose tissue, umbilical cord cutaneous tissue, fetal liver and pulmonary tissue (Rodriguez et al., 2005; Romanov
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br Introduction Free fatty acids FFAs including saturated an
2018-10-22

Introduction Free fatty acids (FFAs), including saturated and unsaturated FFAs, are the major source of lipid energy in human body and released mainly from adipose tissue by lipolysis of triglycerides (Miles and Nelson, 2007). FFAs circulating in the plasma are largely bound to the albumin, with
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The relationship between BCSCs and stem cells in
2018-10-22

The relationship between BCSCs and stem order AM966 in the normal mammary gland has not been defined. Recent studies in mouse mammary glands suggested the existence of separate basal and luminal stem cells. Under normal developmental conditions, each stem cell population gives rise to cells restric
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The advantages of CP could be even more profound for
2018-10-22

The advantages of CP could be even more profound for manufacturing cell therapies. In a typical cell therapy workflow, hPSCs are expanded and banked in a GMP facility before undergoing expensive and time-consuming tests to validate the cell bank. The conversion of this PSC bank into a therapeuticall
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Once we determined that PepC C upregulated CXCL expression
2018-10-22

Once we determined that PepC-C upregulated CXCL12 expression at the injury site and enhanced chemotaxis of NSC from the SVZ, we further investigated if the peptides would modulate the expression of markers for peri-injury reactive astrocytes Gfap, Nestin and Sox2 (Nakagomi et al., 2009; Shimada et a
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The gold standard for determining efficient
2018-10-22

The gold standard for determining efficient HSPC homing to bone marrow niches is their subsequent hematological reconstitution following transplantation in humans (Dahlberg et al., 2011; Ramirez et al., 2012), or, as surrogates, in immunodeficient mice or non-human primates (Goessling et al., 2011;
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The model of this current
2018-10-22

The model of this current study showed that CD133 and CD24 markers, as a single or combined expression, are so promiscuous that they are not able to identify a pure population of adult renal stem cells. In PKHhigh cells, the only cell population presenting self-renew and an in vitro multipotency cap
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In the experiments described in this report molecular and
2018-10-20

In the experiments described in this report, molecular and cellular processes downstream of SOX18 were studied in a non-endothelial background. Effects of SOX18 expression on ASCs were manifested in an endothelial-like cell morphology and responsiveness to forces exerted by laminar flow. SOX18 induc
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Interaction of cadherin cadherin may lead to intercellular a
2018-10-20

Interaction of cadherin–cadherin may lead to intercellular activation of cellular pathways, initiating through lamellipodial protrusions and is followed by the cadherin–catenin–actin cluster formation. The association of cadherin with catenin promotes and stabilizes the AJs, while cisapride polymer
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However our findings confirmed that despite the high levels
2018-10-20

However, our findings confirmed that despite the high levels of cross-engraftment of hematopoietic cells, the analysis of the heart tissue from parabiotic animals, regardless of physical training, revealed very little evidence of extracardiac domoic acid that are targeted to the heart. The same resu
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Human fibroblasts were reprogrammed from an
2018-10-20

Human fibroblasts were reprogrammed from an individual into nociceptor neurons without creation of iPSCs and the neurons exhibited sensitization to the chemotherapeutic drug oxaliplatin, modeling the inherent mechanisms underlying painful CIPN (Wainger et al., 2015); however, the advantage to creati
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br Materials and methods br Acknowledgements This study was
2018-10-20

Materials and methods Acknowledgements This study was supported by the European Union Seventh Framework Programme through funding for the NEUROMICS network (F5-2012-305121 to L.S.), and the DZNE intersite project (grant to L.S.). We thank the patient for participation. Resource table. Reso
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The following are the supplementary data related to this
2018-10-20

The following are the supplementary data related to this article. Acknowledgments The authors wish to thank Dr. K. Inoue for the S100β/GFP-TG rats, Dr. A.F. Parlow and the NIDDK for the Phos-tag against pituitary hormones, Dr. S. Tanaka at Shizuoka University for the antibody against human ACTH
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